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Ethanolamine utilization in Salmonella typhimurium.

D M Roof¹, J R Roth

¹Department of Biology, University of Utah 84112.

Journal of Bacteriology

|September 1, 1988

Summary

Salmonella typhimurium utilizes ethanolamine as a nutrient source when vitamin B12 is present. Researchers mapped genes for ethanolamine utilization and found their expression is regulated by both ethanolamine and vitamin B12.

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Area of Science:

Microbiology
Molecular Biology
Genetics

Background:

Ethanolamine serves as a sole carbon and nitrogen source for Salmonella typhimurium.
Vitamin B12 is essential as a cofactor for ethanolamine utilization.
Understanding the ethanolamine utilization pathway is key to comprehending vitamin B12 synthesis maintenance in S. typhimurium.

Purpose of the Study:

Investigate the regulation of the ethanolamine utilization pathway.
Determine the role of this pathway in maintaining vitamin B12 synthesis.
Identify and characterize mutants defective in ethanolamine utilization.

Main Methods:

Isolation and characterization of ethanolamine utilization (eut) mutants.
Genetic mapping of the eut gene cluster on the Salmonella chromosome.
Construction of eut-lac operon fusions using Mu d lac for transcriptional analysis.

Main Results:

A cluster of eut genes was identified between purC and cysA.
Mutations affecting key enzymes (ethanolamine ammonia lyase, acetaldehyde dehydrogenase) were mapped.
Transcription of eut genes is induced by co-exposure to ethanolamine and vitamin B12.
The eut genes form a single transcriptional unit, with some mutations blocking induction.

Conclusions:

The ethanolamine utilization pathway in S. typhimurium is regulated by both ethanolamine and vitamin B12.
The identified eut genes are crucial for ethanolamine metabolism.
Further study of this pathway can illuminate the selective pressures maintaining vitamin B12 synthesis.

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