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Detection of self-biting behavior of mink by loop-mediated isothermal amplification (LAMP) and sequence-characterized

Z Y Liu1, S S Song1, Z S Huo

  • 1Institute of Special Economic Animal and Plant Sciences, The Chinese Academy of Agricultural Sciences, Jilin Provincial Key Laboratory for Molecular Biology of Special Economic Animals; State Key Laboratory for Molecular Biology of Special Economical Animals, Changchun 130112, China.

Polish Journal of Veterinary Sciences
|November 20, 2018
PubMed

Insights

Self-biting disease in farmed mink can now be rapidly detected using a new molecular marker. This loop-mediated isothermal amplification (LAMP) method offers a more accurate diagnostic tool for this common animal welfare issue.

Area of Science:

  • Animal Genetics
  • Veterinary Diagnostics
  • Molecular Biology

Background:

  • Self-biting disease is a prevalent welfare issue in farmed fur animals globally.
  • Current understanding of its mechanism and rapid detection methods is limited.

Purpose of the Study:

  • To identify a molecular genetic marker linked to the self-biting trait in mink.
  • To develop a rapid and accurate detection method for self-biting disease.

Main Methods:

  • Bulked Segregant Analysis (BSA) combined with RAPD (Random Amplified Polymorphic DNA) was used to find a marker.
  • The identified marker was converted into SCAR (Sequence Characterized Amplified Region) and LAMP (Loop-mediated Isothermal Amplification) assays.
  • Assays were validated on 30 affected and 30 healthy mink.

Main Results:

  • A specific RAPD marker (A10) amplified a 1000bp band in self-biting mink, showing 73% sequence similarity to Canis Brucella.
  • Both SCAR and LAMP markers were developed for this fragment.
  • LAMP demonstrated higher accuracy than SCAR in differentiating between affected and healthy mink (p<0.01 vs p<0.05).

Conclusions:

  • A molecular marker linked to self-biting in mink has been identified.
  • Loop-mediated isothermal amplification (LAMP) provides a rapid, accurate, and effective diagnostic tool for self-biting disease in mink.
Keywords:
loop-mediated isothermal amplificationminkself-bitingsequence-characterized amplified regions

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