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A Gnotobiotic System for Studying Microbiome Assembly in the Phyllosphere and in Vegetable Fermentation
Published on: June 3, 2020
Determination of indole-type phytonutrients in cruciferous vegetables
Panagiota-Kyriaki Revelou1, Maroula G Kokotou1, Violetta Constantinou-Kokotou1
1Department of Food Science and Human Nutrition, Chemical Laboratories, Agricultural University of Athens, Athens, Greece.
Abstract:
Consumption of cruciferous vegetables has been associated with a low risk of developing cancer. Indole-type phytonutrients, derived from enzymatic hydrolysis of glucobrassicin, exhibit cancer-preventive properties and occur in all vegetables of the Brassicaceae family. A LC-Q-TOF-MS methodology was developed and applied in extracts from seven cruciferous vegetables allowing the rapid determination of indole-3-carbinol, indole-3-carbaldehyde, ascorbigen, indole-3-acetic acid and indole-3-acetonitrile. The novel method described herein, was validated and is characterized by low detection limits and excellent linearity. The simultaneous determination of indole-type phytonutrients in turnip and radish was performed for the first time.
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