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Composition of human plaque fluid.

E C Moreno1, H C Margolis

  • 1Forsyth Dental Center, Boston, Massachusetts 02115.

Journal of Dental Research
|September 1, 1988
PubMed
Summary
This summary is machine-generated.

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Dental plaque fluid composition was analyzed in college students. Inorganic and organic acid profiles were determined, revealing differences from saliva and serum, with organic acids primarily responsible for buffering capacity.

Area of Science:

  • Oral biology
  • Biochemistry
  • Dental research

Background:

  • Understanding dental plaque fluid composition is crucial for oral health research.
  • Previous studies have provided limited data on the inorganic and organic constituents of plaque fluid.

Purpose of the Study:

  • To determine the inorganic and organic composition of pooled resting plaque fluid.
  • To compare plaque fluid composition with saliva and serum.
  • To investigate the buffering capacity of plaque fluid.

Main Methods:

  • Collected pooled resting plaque fluid from four groups of 50 college-age students.
  • Processed samples via centrifugation under mineral oil.
  • Analyzed inorganic ions (Ca, P, Na, K, Mg, Cl, F) and organic acids (acetate, propionate, etc.).

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  • Assessed buffering capacity through titration.
  • Main Results:

    • Plaque fluid composition differed significantly from saliva and serum.
    • Mean inorganic composition: Ca (7.07 mmol/L), P (23.2 mmol/L), Na (18.6 mmol/L), K (85.1 mmol/L), Mg (3.9 mmol/L), Cl (42.8 mmol/L), F (~0.004 mmol/L), pH (5.69).
    • Acetate and propionate were the predominant organic acids; they were the main contributors to buffering capacity, especially at lower pH.

    Conclusions:

    • Dental plaque fluid has a distinct inorganic and organic composition compared to saliva and serum.
    • Organic acids, primarily acetate and propionate, are key to plaque fluid's buffering capacity.
    • Phosphate contributes to buffering when plaque fluid pH exceeds 6.5.