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Partial characterization of antigens from M. leprae evoking IgG and IgM antibodies in armadillos
A R Vadiee1, E J Shannon, T P Gillis
1Immunology Research Department, GWL Hansen's Disease Center, Carville, Louisiana 70721.
Armadillo IgG and IgM antibody responses to Mycobacterium leprae were analyzed using isotypic-specific antisera by means of immunoblotting. Blots developed for IgG antibodies to M. leprae showed multiple protein antigens (Mr = 12-90 K) in some heavily infected armadillos. In contrast, blots developed for IgM antibodies to M. leprae showed a single, broad, diffuse band of immunoreactivity at approximately 33 kDa. The 33-kDa immunogen was detectable with silver stain modified for carbohydrate reactivity, suggesting the presence of a polysaccharide component. In addition, binding of 125I-concanavalin A to the 33-kDa component demonstrated the presence of mannose and/or glucose residues.
Armadillo IgG and IgM antibody responses to Mycobacterium leprae were analyzed using isotypic-specific antisera by means of immunoblotting. Blots developed for IgG antibodies to M. leprae showed multiple protein antigens (Mr = 12-90 K) in some heavily infected armadillos. In contrast, blots developed for IgM antibodies to M. leprae showed a single, broad, diffuse band of immunoreactivity at approximately 33 kDa. The 33-kDa immunogen was detectable with silver stain modified for carbohydrate reactivity, suggesting the presence of a polysaccharide component. In addition, binding of 125I-concanavalin A to the 33-kDa component demonstrated the presence of mannose and/or glucose residues.