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Spen limits intestinal stem cell self-renewal.

Maheva Andriatsilavo1, Marine Stefanutti1, Katarzyna Siudeja1

  • 1Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934, Stem Cells and Tissue Homeostasis group, Sorbonne Université, UPMC Univ Paris 6, Paris, France.

Plos Genetics
|November 20, 2018
PubMed
Summary
This summary is machine-generated.

The split-ends (spen) gene regulates adult stem cell fate in the Drosophila intestine. It controls stem cell commitment and limits proliferation, offering new insights into Spen protein functions.

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Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Genetics

Background:

  • Precise regulation of stem cell self-renewal and differentiation is crucial for tissue homeostasis.
  • The adult Drosophila intestine serves as a model system for studying stem cell regulation.

Purpose of the Study:

  • To identify novel regulators of intestinal stem cell (ISC) fate in Drosophila.
  • To elucidate the role of the split-ends (spen) gene in ISC self-renewal and differentiation.

Main Methods:

  • Genetic screening in adult Drosophila intestine.
  • Two-color cell sorting of stem cells and their progeny.
  • Analysis of RNA abundance and exon usage.

Main Results:

  • The gene split-ends (spen) was identified as a novel regulator of ISC fate.
  • spen functions intrinsically within ISCs to control early commitment events.
  • spen in differentiated cells limits ISC proliferation.
  • spen-dependent changes in RNA abundance and exon usage were characterized.

Conclusions:

  • spen is a critical regulator of adult stem cell function in the Drosophila intestine.
  • This study provides new insights into the functions of Spen-family proteins.
  • The findings may illuminate Spen's role in other developmental contexts.