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An Automated Algorithm to Quantify Collagen Distribution in Aortic Wall.

Dustin M Nguyen1, Markus U Wagenhäuser2,3, Dennis Mehrkens4

  • 1Department of Chemical and Materials Engineering, San José State University, San José, CA.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|November 20, 2018
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Summary
This summary is machine-generated.

This study introduces an automated algorithm for precisely measuring collagen in mouse aorta layers. This method enhances the analysis of arterial diseases like atherosclerosis and aortic aneurysms.

Keywords:
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Area of Science:

  • Biomedical Engineering
  • Histology
  • Cardiovascular Research

Background:

  • Arterial diseases such as abdominal aortic aneurysm and atherosclerosis are biomechanical conditions.
  • Changes in collagen and elastin matrix turnover significantly impact arterial stiffening and disease progression.
  • Current methods for quantifying aortic collagen content are often imprecise due to difficulties in separating medial and adventitial layers.

Purpose of the Study:

  • To develop an automated algorithm for layer-specific collagen quantification in mouse aortas.
  • To accurately measure collagen content in the media and adventitia separately using histological images.
  • To provide a rapid and reliable tool for analyzing collagen distribution in connective tissues.

Main Methods:

  • Developed an algorithm for automatic quantification of layer-specific collagen content.
  • Utilized bright-field and polarized microscopic images of Picrosirius red (PSR) stained mouse aorta.
  • Image processing involved layer separation, erosion, region segregation, binarization, and pixel intensity quantification.

Main Results:

  • The automated algorithm accurately quantified collagen content in both medial and adventitial layers.
  • The method demonstrated rapid and reliable quantification across a range of image qualities.
  • Performance was particularly notable in accurately measuring collagen where medial and adventitial layers overlapped.

Conclusions:

  • The developed algorithm enables precise, automated, layer-specific collagen quantification in histological sections.
  • This tool significantly impacts the analysis of collagen distribution in arterial diseases.
  • The algorithm offers a rapid, reliable, and accurate method for connective tissue analysis.