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Infecting Mice with Malassezia spp. to Study the Fungus-Host Interaction
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A Ketoconazole Susceptibility Test for Malassezia pachydermatis Using Modified Leeming⁻Notman Agar
Bo-Young Hsieh1,2, Wei-Hsun Chao3, Yi-Jing Xue4
1Lugu Township Administration, Nanto County 55844, Taiwan. Qwerkboy@gmail.com.
Journal of Fungi (Basel, Switzerland)
|November 21, 2018
Summary
A new ketoconazole susceptibility test for Malassezia pachydermatis offers a cost-effective and efficient alternative. This novel disk diffusion method provides reliable minimum inhibitory concentration (MIC) values, simplifying antifungal susceptibility testing.
Area of Science:
- Mycology
- Antimicrobial Susceptibility Testing
- Veterinary Dermatology
Background:
- Malassezia pachydermatis is a yeast commonly associated with dermatological conditions in animals.
- Accurate susceptibility testing is crucial for effective antifungal treatment.
- Existing methods for determining ketoconazole susceptibility can be time-consuming and technically demanding.
Purpose of the Study:
- To develop and validate a novel ketoconazole susceptibility test for Malassezia pachydermatis.
- To compare the efficacy of a new disk diffusion method with established methods for determining minimum inhibitory concentration (MIC).
- To establish a cost-effective and time-efficient alternative for antifungal susceptibility testing.
Main Methods:
- Modified Leeming-Notman agar (mLNA) was used as the growth medium.
- A modified CLSI M27-A3 method was employed for comparison.
- A disk diffusion method utilizing varying concentrations of ketoconazole on paper disks was developed.
- Susceptibility testing was performed on 33 Malassezia pachydermatis isolates.
Main Results:
- The novel disk diffusion test demonstrated high concordance with the modified CLSI M27-A3 method, with 93.9% of minimum inhibitory concentration (MIC) values showing agreement within two dilutions.
- Standardized strains, Malassezia pachydermatis BCRC 21676 and Candida parapsilosis ATCC 22019, were used for verification and confirmed consistency between methods.
- The developed test proved to be a reliable indicator of ketoconazole susceptibility.
Conclusions:
- The novel disk diffusion test is a valid, cost-effective, and time-efficient method for determining ketoconazole susceptibility in Malassezia pachydermatis.
- This method is less technically demanding than existing protocols, making it accessible for broader laboratory application.
- The findings support the adoption of this new test for routine antifungal susceptibility diagnostics.
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