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Induction of Retinal Ischemia-Reperfusion Injury in a Mouse Eye Model
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Induction of Retinal Ischemia-Reperfusion Injury in a Mouse Eye Model

Published on: December 20, 2024

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Involvement of HDAC6 in ischaemia and reperfusion-induced rat retinal injury

Haihong Yuan1, Hui Li2,3, Ping Yu2

  • 1Department of Pharmacy, Shanghai University of Medicine & Health Science, Shanghai, China.

BMC Ophthalmology
|November 21, 2018
PubMed

Insights

Histone deacetylase 6 (HDAC6) inhibition protects retinal cells from injury. Tubacin, an HDAC6 inhibitor, reduced cell death and promoted survival in a retinal ischemia-reperfusion injury model, suggesting a therapeutic target for glaucoma.

Area of Science:

  • Neuroscience
  • Ophthalmology
  • Molecular Biology

Background:

  • Histone deacetylases 6 (HDAC6) are implicated in neurodegeneration.
  • The role of HDAC6 in retinal degenerative conditions is largely unknown.
  • Retinal ischemia-reperfusion (I/R) injury models provide insights into neurodegenerative processes.

Purpose of the Study:

  • To investigate the role of HDAC6 in retinal I/R injury.
  • To evaluate the therapeutic potential of HDAC6 inhibition in retinal neurodegeneration.

Main Methods:

  • Retinal pathology assessed using H&E staining.
  • HDAC6 expression and activity measured via Western blotting and colorimetric assays.
  • Apoptosis and autophagy markers quantified using Western blotting and RT-PCR.

Main Results:

  • HDAC6 inhibition with tubacin ameliorated I/R-induced retinal thinning and increased retinal ganglion cell (RGC) survival.
  • Tubacin attenuated I/R-mediated apoptosis and enhanced autophagy-related protein expression.
  • The protective effects of tubacin were linked to acetylated α-tubulin.

Conclusions:

  • Tubacin demonstrates neuroprotective effects in a model of retinal I/R injury.
  • HDAC6 inhibition represents a promising therapeutic strategy for glaucoma and other retinal neurodegenerative diseases.
Abstract

Keywords:
GlaucomaHDAC6Ischaemia and reperfusionNeuroprotectionTubacin

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