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Updated: Feb 2, 2026

Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue
Published on: May 28, 2015
Cell-type-specific and projection-specific brain-wide reconstruction of single neurons
Rui Lin1,2, Ruiyu Wang1,2, Jing Yuan3,4,5
1School of Life Sciences, Peking University, Beijing, China.
Abstract:
We developed a dual-adeno-associated-virus expression system that enables strong and sparse labeling of individual neurons with cell-type and projection specificity. We demonstrated its utility for whole-brain reconstruction of midbrain dopamine neurons and striatum-projecting cortical neurons. We further extended the labeling method for rapid reconstruction in cleared thick brain sections and simultaneous dual-color labeling. This labeling system may facilitate the process of generating mesoscale single-neuron projectomes of mammalian brains.
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