Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Molecular Chaperones and Protein Folding03:00

Molecular Chaperones and Protein Folding

19.8K
The native conformation of a protein is formed by interactions between the side chains of its constituent amino acids. When the amino acids cannot form these interactions, the protein cannot fold by itself and needs chaperones. Notably, chaperones do not relay any additional information required for the folding of polypeptides; the native conformation of a protein is determined solely by its amino acid sequence. Chaperones catalyze protein folding without being a part of the folded protein.
The...
19.8K
Molecular Chaperones and Protein Folding03:00

Molecular Chaperones and Protein Folding

15.0K
15.0K
What is the Cell Cycle?00:56

What is the Cell Cycle?

10.5K
The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: the interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the...
10.5K
What is the Cell Cycle?01:04

What is the Cell Cycle?

242.9K
The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...
242.9K
The Cell Cycle Control System01:28

The Cell Cycle Control System

5.6K
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
5.6K
The Cell Cycle Control System02:11

The Cell Cycle Control System

14.3K
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
14.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early neutrophil infiltration promotes TRIMELVax-induced antitumor immunity by linking local inflammation to tumor control.

Oncoimmunology·2026
Same author

Comparative biodegradation of functionalized graphene oxide nanosheets by myeloperoxidase and neutrophil extracellular traps.

Frontiers in bioengineering and biotechnology·2026
Same author

Fragmentation-Induced Disassembly and Reaggregation of α-Synuclein Amyloid Fibrils.

ACS chemical neuroscience·2026
Same author

Lonp1 inhibition disrupts mitochondrial function in the hippocampus and drives an aging-like synaptic and cognitive phenotype in adult SAMP8 mice.

Free radical biology & medicine·2026
Same author

Early mitophagy activation by Urolithin A prevents, but late activation does not reverse, age-related cognitive impairment.

npj aging·2026
Same author

Heat shock protein 10 as a chaperone modulating α-synuclein amyloid fibril formation.

Protein science : a publication of the Protein Society·2026

Related Experiment Video

Updated: Feb 2, 2026

Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions
06:55

Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions

Published on: June 7, 2020

3.3K

Copper Chaperone Atox1 Interacts with Cell Cycle Proteins.

Maria Matson Dzebo1, Stéphanie Blockhuys1, Sebastian Valenzuela1

  • 1Department of Biology and Biological Engineering, Chalmers University of Technology, 412 96 Gothenburg, Sweden.

Computational and Structural Biotechnology Journal
|November 21, 2018
PubMed
Summary

The copper chaperone Atox1 interacts with the anaphase-promoting complex (APC), a key regulator of cell division. Atox1 deficiency slows cell proliferation, suggesting a novel role for Atox1 in cell cycle modulation.

More Related Videos

Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay
06:51

Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay

Published on: July 21, 2021

3.2K
In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays
08:16

In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays

Published on: December 29, 2021

3.2K

Related Experiment Videos

Last Updated: Feb 2, 2026

Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions
06:55

Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions

Published on: June 7, 2020

3.3K
Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay
06:51

Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay

Published on: July 21, 2021

3.2K
In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays
08:16

In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays

Published on: December 29, 2021

3.2K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The anaphase-promoting complex (APC) is crucial for cell cycle progression, particularly sister chromatid separation during mitosis.
  • APC is a validated target for anticancer drug development.
  • Atox1 is a copper chaperone involved in intracellular copper transport.

Purpose of the Study:

  • To investigate the potential interaction between the copper chaperone Atox1 and the anaphase-promoting complex (APC).
  • To determine the role of Atox1 in cell cycle regulation.
  • To explore Atox1 as a potential target for anticancer therapies.

Main Methods:

  • Co-immunoprecipitation followed by mass spectrometry to identify Atox1-APC interactions.
  • Proximity ligation assays in HEK293T cells to confirm interactions.
  • Comparison of cell cycle progression and proliferation rates in wild-type versus Atox1-knockout cells.

Main Results:

  • Atox1 was found to interact with multiple subunits of the anaphase-promoting complex (APC).
  • Atox1 knockout cells exhibited prolonged G2/M phases.
  • Atox1 deficiency led to a reduced cellular proliferation rate.

Conclusions:

  • Atox1 interacts with the anaphase-promoting complex (APC), suggesting a role beyond copper transport.
  • Atox1 influences cell cycle progression, potentially by modulating APC activity.
  • Atox1 represents a novel target for modulating cell cycle control in cancer therapy.