Related Experiment Video
Updated: Feb 2, 2026

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins
Published on: July 2, 2010
Azide-tagged sphingolipids for the proteome-wide identification of C16-ceramide-binding proteins
Janine Schulte-Zweckel1, Tabea Schneidewind, Jose Luis Abad
1Department of Chemical Biology, Max-Planck-Institute of molecular Physiology, Otto-Hahn-Strasse 11, D-44227 Dortmund, Germany.
Abstract:
Ceramide plays key roles in autophagy, inflammation and apoptosis. However, little is known about the molecular mechanisms regulating its function and only a handful of cellular effectors are known for this lipid. Here we show that azide-tagged sphingolipids are powerful tools to identify ceramide targets. The combination of a protein array analysis and a mass spectrometry-based proteomic profiling successfully detects known ceramide-binding proteins and identifies others not yet reported, several of which we validated using a variety of techniques.
More Related Videos
Related Concept Videos
Tagging and Fusion Proteins
Conserved Binding Sites
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
Factors Affecting Protein-Drug Binding: Protein-Related Factors
The physicochemical properties of a drug play a significant role in its ability to bind to proteins. Lipophilic drugs, which dissolve in fats, oils, and lipids, can be...
The Equilibrium Binding Constant and Binding Strength
Drug Distribution: Plasma Protein Binding
Protein-Drug Binding: Determination Methods
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...

