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Function of monocytes in patients with systemic sclerosis.

L Czirják¹, K Dankó, M Zeher

¹3rd Department of Medicine, University Medical School of Debrecen, Hungary.

Acta Medica Hungarica

|January 1, 1988

Summary

Monocytes in systemic sclerosis patients show normal phagocytosis but reduced chemotaxis. However, this chemotactic deficit resolves with in vitro culture, suggesting it

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Area of Science:

Immunology
Rheumatology

Background:

Systemic sclerosis (SSc) is an autoimmune disease characterized by fibrosis.
Monocyte dysfunction may contribute to SSc pathogenesis.

Purpose of the Study:

To investigate the in vitro functions of peripheral blood monocytes in patients with systemic sclerosis.
To assess monocyte phagocytosis, antibody-dependent cell-mediated cytotoxicity (ADCC), and chemotaxis in SSc patients.

Main Methods:

Monocyte functions including yeast phagocytosis, opsonized yeast phagocytosis, and erythrocyte-antibody (EA) binding were assessed in vitro.
Chemotactic responses to a zymosan-activated, complement-derived factor were evaluated.
Monocyte cultures were maintained for 168 hours to assess changes in chemotactic activity over time.

Main Results:

Monocytes from SSc patients exhibited normal yeast phagocytosis, opsonized yeast phagocytosis, and EA binding.
A depressed chemotactic response was observed in SSc monocytes.
Monocyte chemotaxis normalized after 5-7 days of in vitro culture.

Conclusions:

The observed chemotactic defect in systemic sclerosis monocytes is likely not due to an intrinsic abnormality.
These findings suggest a transient or environmentally-induced impairment of monocyte chemotaxis in SSc.

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