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3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
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3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

Published on: September 13, 2018

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Invasion-related circular RNA circFNDC3B inhibits bladder cancer progression through the miR-1178-3p/G3BP2/SRC/FAK

Hongwei Liu1, Junming Bi1, Wei Dong1

  • 1Department of Urology and Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107th Yanjiangxi Road, Yuexiu District, Guangzhou, 510120, China.

Molecular Cancer
|November 22, 2018
PubMed
Abstract

Insights

Circular RNA FNDC3B (circFNDC3B) is downregulated in bladder cancer (BC) and acts as a tumor suppressor. Restoring circFNDC3B inhibits BC cell growth and metastasis by sponging miR-1178-3p and suppressing the G3BP2 oncogene.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are increasingly recognized for their roles in cancer.
  • The specific function and regulatory mechanisms of circFNDC3B in bladder cancer (BC) are currently unknown.

Purpose of the Study:

  • To investigate the role and regulatory mechanisms of circFNDC3B in bladder cancer.
  • To identify circRNAs involved in BC cell invasion.

Main Methods:

  • Established a BC cell invasion model using transwell assays.
  • Quantified circFNDC3B expression in BC tissues and cell lines via qRT-PCR.
  • Performed in vitro and in vivo functional assays to assess circFNDC3B's impact on tumor progression.
  • Utilized FISH, pull-down, and luciferase assays to confirm the interaction between circFNDC3B and miR-1178-3p.

Main Results:

  • Identified circFNDC3B as a novel, downregulated circRNA in BC tissues, correlating with advanced pathological T stage, grade, lymphatic invasion, and poorer survival.
  • Overexpression of circFNDC3B significantly inhibited BC cell proliferation, migration, and invasion in vitro and suppressed tumor growth and metastasis in vivo.
  • Demonstrated that circFNDC3B acts as a molecular sponge for miR-1178-3p, targeting the 5'UTR of the oncogene G3BP2 and inhibiting the SRC/FAK signaling pathway.

Conclusions:

  • circFNDC3B functions as a tumor suppressive factor in bladder cancer.
  • circFNDC3B represents a potential therapeutic target for human BC.
Keywords:
Bladder cancerG3BP2SRC/FAKcircFNDC3BmiR-1178-3p

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