Ultraviolet (IUV) and mass spectrometry (IMS) imaging for the deconvolution of microbial interactions
Víctor González-Menéndez1, Germán Martínez1, Rachel Serrano1
1Fundación MEDINA, Avda Conocimiento 34, CDFA, PTS, 18016, Granada, Spain.
Background:
Spatial localization of natural products or proteins during microbial interactions can help to identify new antimicrobials both as offensive or defensive agents. Visible spatial interactions have been used for decades to enhance Drug Discovery processes both in industry and academia.
Results:
Herein we describe an automated micro-extraction methodology, that coupled with the previously described HPLC-Studio 2.0 software and the new development, the MASS-Studio 1.0 software, can combine multiple chemical analyses to generate ultraviolet (UV) and mass spectrometry (MS) images from traditional affordable analytical equipment. As a proof of concept, we applied this methodology on two microbial antagonisms observed among co-habitant endophytes isolated from endemic plants of arid areas of the south of Europe.
Conclusions:
The use of UV and MS images highlighted interacting naturals products and allowed clear identification of induced molecules of interest not produced by the strains when cultured individually.
Related Concept Videos
Deconvolution
Deconvolution involves several mathematical techniques to derive the impulse response. One common approach is polynomial division. In this method, the input and output sequences are treated as coefficients of...
Mass Spectrometry: Overview
Tandem Mass Spectrometry
Mass Spectrometry of Amines
Mass Spectrometry: Isotope Effect
Chemical Ionization (CI) Mass Spectrometry


