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Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages
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Isolation and characterization of ovine monocyte-derived macrophages from peripheral blood

Yongxiao Liu1, Dongbing Yang1, Shao Wei1

  • 1Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China; National Engineering Laboratory For Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.

Veterinary Immunology and Immunopathology
|November 22, 2018
PubMed

Insights

Ovine peripheral blood macrophages were established and characterized. These macrophages exhibit M2-like properties, suppressing inflammation and showing reduced phagocytic activity compared to other macrophage types.

Area of Science:

  • Veterinary Immunology
  • Cell Biology

Background:

  • Macrophages are crucial for host defense against intracellular pathogens.
  • Ovine peripheral blood monocyte-derived cell lines are established but lack detailed characterization.
  • Understanding ovine macrophage phenotypes is essential for immunology research.

Purpose of the Study:

  • To establish and characterize novel ovine macrophage cell lines from peripheral blood.
  • To compare the phenotypic and functional properties of blood macrophages (BMs) with alveolar macrophages (AMs) and splenic macrophages (SMs).
  • To investigate gene expression profiles related to macrophage polarization.

Main Methods:

  • Establishment of spontaneous, long-term proliferating ovine macrophage cell lines from peripheral blood adherent cells.
  • Assessment of macrophage characteristics including morphology, adhesion, cell surface marker expression, phagocytic activity, and inflammatory response.
  • Comparative gene expression analysis of BMs versus AMs and SMs for membrane proteins, cytokines, and chemokines.
  • Evaluation of M1 and M2 macrophage marker gene expression (iNOS, IDO, ARG2, TGFβ1).

Main Results:

  • Ovine blood macrophages (BMs) exhibited distinct characteristics compared to AMs and SMs.
  • BMs showed lower expression of CD11b, CD80, IL-1β, TNFα, IL-8/CXCL8, and CCL-21, but not CD200.
  • BMs displayed reduced M1 markers (iNOS, IDO) and increased M2 markers (ARG2, TGFβ1), lower phagocytic ability, and higher Salmonella proliferation.
  • BMs demonstrated suppressed inflammatory responses and M2-like phenotypic traits.

Conclusions:

  • Established ovine blood macrophage cell lines provide a valuable in vitro model.
  • BMs possess characteristics of M2-polarized macrophages, contributing to immune suppression.
  • These findings advance the understanding of ovine innate immunity and macrophage heterogeneity.
Keywords:
Alveolar macrophagesOvine macrophagePeripheral bloodSplenic macrophages

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