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Revealing lncRNA Structures and Interactions by Sequencing-Based Approaches
Xingyang Qian1, Jieyu Zhao2, Pui Yan Yeung2
1MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China; These authors contributed equally to this work.
Trends in Biochemical Sciences
|November 22, 2018
Summary
Long noncoding RNAs (lncRNAs) are key regulators of gene function. New sequencing methods reveal their complex structures and interactions, advancing our understanding of their roles in health and disease.
Area of Science:
- Molecular Biology
- Genomics
- Biochemistry
Background:
- Long noncoding RNAs (lncRNAs) play critical roles in gene regulation.
- Understanding lncRNA structure and interactions is vital for elucidating their cellular functions.
Purpose of the Study:
- To review current sequencing strategies for mapping RNA structure and interactions.
- To highlight applications of these methods in identifying functional lncRNAs.
Main Methods:
- Combination of biochemical approaches with automated capillary and high-throughput sequencing (HTS).
- Analysis of RNA structure, RNA-RNA, RNA-DNA, and RNA-protein interactions.
Main Results:
- Revealed the complexity and diversity of RNA structures and interactions across species.
- Uncovered biological insights into lncRNA functions in gene expression, RNA metabolism, development, and disease.
Conclusions:
- Advanced sequencing strategies are crucial for mapping functional lncRNAs.
- Further methodological development is needed to fully decipher lncRNA mechanisms and disease implications.
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