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Author Correction: Comprehensive comparative analysis of 5'-end RNA-sequencing methods.

Xian Adiconis^1,2, Adam L Haber¹, Sean K Simmons²

¹Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.

|November 22, 2018

Summary

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A correction has been issued for a previously published paper regarding a specific primer sequence used in Rampage library preparation. The corrected sequence ensures accurate methodology for future research.

Area of Science:

Molecular Biology
Genomics
Biotechnology

Context:

Ensuring the accuracy of published scientific data is crucial for reproducible research.
Methodological details, such as primer sequences, are fundamental to experimental success.

Purpose:

To correct an error in the reported primer sequence for Rampage library indexing.
To provide the accurate oligonucleotide sequence for future reference and replication.

Summary:

The original publication incorrectly stated a reverse primer sequence for library indexing in the Rampage libraries method.
The corrected sequence, 5 -CAAGCAGAAGACGGCATACGAGATXXXXXXXXGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3 , has been updated in the official versions of the paper.

Impact:

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This correction ensures the integrity of the scientific record.
Facilitates accurate implementation of the Rampage library preparation protocol by other researchers.
Upholds standards of scientific rigor and transparency in molecular biology research.