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MicroRNA-129-5p inhibits 3T3-L1 preadipocyte proliferation by targeting G3BP1

Shun Lv1, Meilin Ma1, Yunmei Sun1

  • 1Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi Province, People's Republic of China.

Animal Cells and Systems
|November 22, 2018
PubMed

Insights

MicroRNA-129-5p suppresses preadipocyte proliferation by targeting G3BP1. This microRNA (miRNA) also activates the p38 signaling pathway, revealing a novel mechanism in cell growth regulation.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are key regulators of cellular processes, including cell proliferation.
  • Preadipocyte proliferation is a critical step in adipogenesis and is influenced by various molecular factors.

Purpose of the Study:

  • To investigate the role of miR-129-5p in 3T3-L1 preadipocyte proliferation.
  • To identify the molecular targets and signaling pathways regulated by miR-129-5p in preadipocytes.

Main Methods:

  • Quantitative reverse transcription PCR (qRT-PCR) to measure miRNA and gene expression.
  • 5-Ethynyl-2'-deoxyuridine (EdU) incorporation assay and flow cytometry to assess cell proliferation and cell cycle.
  • Western blotting to analyze protein expression and signaling pathway activation.
  • Luciferase reporter assay to confirm direct targeting of G3BP1 by miR-129-5p.

Main Results:

  • miR-129-5p expression was downregulated, while G3BP1 expression was upregulated during 3T3-L1 preadipocyte proliferation.
  • Overexpression of miR-129-5p reduced S-phase cells, induced G2-phase arrest, and impaired cell cycle-related genes.
  • miR-129-5p directly targeted the 3' untranslated region (3'UTR) of G3BP1, inhibiting its expression.
  • miR-129-5p mimic activated the p38 signaling pathway by upregulating p38 and its phosphorylation.

Conclusions:

  • miR-129-5p acts as a suppressor of 3T3-L1 preadipocyte proliferation.
  • The inhibitory effect of miR-129-5p on proliferation is mediated through direct targeting of G3BP1 and activation of the p38 signaling pathway.
Keywords:
3T3-L1 preadipocyteG3BP1MiR-129-5pcell proliferationp38 signaling pathway

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