Related Experiment Video
Updated: Feb 2, 2026

Small-Scale Extraction of Caenorhabditis elegans Genomic DNA
Published on: June 7, 2022
VIG-1 is required for maintenance of genome stability in Caenorhabditis elegans
Bala Murali Krishna Vasamsetti1, Yang-Seo Park1, Nam Jeong Cho1
1Department of Biochemistry, College of Natural Sciences, Chungbuk National University, Cheongju, South Korea.
Abstract:
To explore the function of VIG-1 in Caenorhabditis elegans, we analyzed the phenotypes of two vig-1 deletion mutants: vig-1(tm3383) and vig-1(ok2536). Both vig-1 mutants exhibited phenotypes associated with genome instability, such as a high incidence of males (Him) and increased embryonic lethality. These phenotypes became more evident in succeeding generations, implying that the germline of vig-1 accumulates DNA damage over generations. To examine whether vig-1 causes a defect in the DNA damage response, we treated worms with UV or camptothecin, a specific topoisomerase I inhibitor. We observed that the embryonic survival of the vig-1 mutants was reduced compared with that of the wild-type worms. Our results thus suggest that VIG-1 is required for maintaining genome stability in response to endogenous and exogenous genotoxic stresses.
Related Concept Videos
Nuclear Stability
To hold positively charged protons together...
Genomics
Requirements for Human Life
Oxygen
Atmospheric air is only about 20 percent oxygen, but that oxygen is a key component of the chemical reactions that keep the body alive, including the reactions that produce ATP. Brain cells are susceptible to a lack of oxygen because they require a...
RNA Stability
Maintenance of the ES Cell State
Stability
The stability of an LTI system is determined by the roots of its characteristic equation, known as poles. A system is stable if it produces a bounded...

