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Updated: Feb 2, 2026

Imaging and Analysis for Quantifying Maize (Zea mays) Abiotic Stress Phenotypes
Published on: March 28, 2025
Methods for the Simple and Reliable Assessment of Barley Sensitivity to Abiotic Stresses During Early Development
Agata Daszkowska-Golec1, Anna Skubacz2, Marzena Kurowska2
1Department of Genetics, Faculty of Biology and Environmental Protection, University of Silesia in Katowice, Katowice, Poland. agata.daszkowska@gmail.com.
Abstract:
Physiological assays that facilitate screening for various types of responses to abiotic stresses are well established for model plants such as Arabidopsis; however, there is a need to optimize similar tests for cereal crops, including barley. We have developed a set of stress assays to characterize the response of different barley lines during two stages of development-seed germination and seedling growth. The assays presented, including the response to osmotic, salt, oxidative stresses, and exogenously applied abscisic acid, can be used for forward screening of populations after mutagenesis as well as for phenotyping of already isolated mutants, cultivars, or breeding lines. As well as protocols for stress treatments, we also provide methods for plant stress response evaluation, such as chlorophyll a fluorescence (ChlF) and image analysis.
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