[Localization of neutral Mn-dependent DNAse in rat hepatocytes. An immunofluorescent study]

Rabbit antibodies against the Mn-dependent DNAse were obtained. The specificity of the anti-DNAse antibody was established by the enzyme inhibition in vitro. The enzyme activity was inhibited by more than 75 and 54% using rabbit antisera and affinity-purified IgG. Localization of the enzyme in the rat hepatocyte nuclei was studied by indirect immunofluorescence.