Related Experiment Video
Updated: Feb 2, 2026
08:48
Writing Bragg Gratings in Multicore Fibers
Published on: April 20, 2016
8.7K
Immunosensing platform with large detection range using an excessively tilted fiber grating coated with graphene
Applied Optics
|November 22, 2018
Summary
This study introduces a novel graphene oxide-coated fiber optic biosensor for detecting Bovine Serum Albumin (BSA). The developed immunosensing platform offers a wide detection range and rapid response, paving the way for sensitive biomolecule detection.
Area of Science:
- Optoelectronics
- Biosensing
- Materials Science
Background:
- Fiber optic sensors offer label-free detection capabilities.
- Graphene oxide (GO) possesses unique optical and electrical properties suitable for biosensing applications.
- Excessively tilted fiber gratings (ExTFGs) provide enhanced sensitivity for optical interrogation.
Purpose of the Study:
- To develop and characterize a novel immunosensing platform utilizing an ExTFG coated with GO.
- To evaluate the performance of the GO-coated ExTFG biosensor for the detection of Bovine Serum Albumin (BSA).
- To demonstrate the potential of this platform for sensitive and specific detection of biomolecules.
Main Methods:
- Fabrication of an ExTFG inscribed in standard single-mode fiber.
- Coating the ExTFG surface with graphene oxide (GO) via hydrogen bonding.
- Immobilization of BSA-specific monoclonal antibodies (MAbs) onto the GO layer through covalent bonding.
- Detection of BSA using spectral evolution analysis of the sensor's optical response.
Main Results:
- The GO-coated ExTFG biosensor exhibited a broad detection range for BSA, from 1.5 nM to 75 nM.
- A low limit of detection (LOD) of approximately 0.88 nM was achieved.
- The sensor demonstrated a fast response time and high sensitivity, with calculated dissociation (KD) and affinity (KA) constants of ~6.66×10-9 M and ~1.5×108 M-1, respectively.
Conclusions:
- The developed GO-coated ExTFG immunosensing platform is effective for sensitive and specific detection of BSA.
- The platform demonstrates a large dynamic range and fast response, suitable for various biosensing applications.
- This technology provides a foundation for the development of advanced biosensors for detecting other target biomolecules.
Related Concept Videos
00:59Range
14.2K
The range is one of the measures of variation. It can be defined as the difference between a dataset's highest and lowest values. For example, in the study of seven 16-ounce soda cans, the filled volume of soda was measured, thus producing the following amount (in ounces) of soda:
15.9; 16.1; 15.2; 14.8; 15.8; 15.9; 16.0; 15.5
Measurements of the amount of soda in a 16-ounce can vary since different subjects record these measurements or since the exact amount - 16 ounces of liquid, was not...
15.9; 16.1; 15.2; 14.8; 15.8; 15.9; 16.0; 15.5
Measurements of the amount of soda in a 16-ounce can vary since different subjects record these measurements or since the exact amount - 16 ounces of liquid, was not...
14.2K
01:48Classification of Skeletal Muscle Fibers
59.5K
Skeletal muscles continuously produce ATP to provide the energy that enables muscle contractions. Skeletal muscle fibers can be categorized into three types based on differences in their contraction speed and how they produce ATP, as well as physical differences related to these factors. Most human muscles contain all three muscle fiber types, albeit in varying proportions.
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
59.5K
03:14Oxidation Numbers
42.6K
In redox reactions, the transfer of electrons occurs between reacting species. Electron transfer is described by a hypothetical number called the oxidation number (or oxidation state). It represents the effective charge of an atom or element, which is assigned using a set of rules.
42.6K
01:13Excess Pressure Inside a Drop and a Bubble
3.5K
The shape of a small drop of liquid can be considered spherical, neglecting the effect of gravity. This drop can further be considered as two equal hemispherical drops put together due to surface tension. The forces acting on the spherical drop are due to the pressure of the liquid inside the drop, the pressure due to air outside the drop, and the force due to the surface tension acting on the two hemispherical drops.
3.5K
01:15Pyruvate Oxidation
168.8K
After glycolysis, the charged pyruvate molecules enter the mitochondria via active transport and undergo three enzymatic reactions. These reactions ensure that pyruvate can enter the next metabolic pathway so that energy stored in the pyruvate molecules can be harnessed by the cells.
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
168.8K
01:27¹H NMR: Long-Range Coupling
2.7K
The coupling interactions of nuclei across four or more bonds are usually weak, with J values less than 1 Hz. While these are usually not observed in spectra, the presence of multiple bonds along the coupling pathway can result in observable long-range coupling.
In alkenes, spin information is communicated via σ–π overlap, as seen in allylic (four-bond) and homoallylic (five-bond) couplings. These coupling interactions are stronger when the σ bond is parallel to the alkene...
In alkenes, spin information is communicated via σ–π overlap, as seen in allylic (four-bond) and homoallylic (five-bond) couplings. These coupling interactions are stronger when the σ bond is parallel to the alkene...
2.7K