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Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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The alternative coordinate method, also known as the Shoelace Formula, is a technique for determining the area of a traverse using Cartesian coordinates. This method relies on the sequential arrangement of x and y coordinates for each point of the shape, ensuring accuracy and ease of application.In this approach, each corner's x and y coordinates are listed as fractions, with the x-coordinate as the numerator and the y-coordinate as the denominator. These coordinates are arranged sequentially...
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Internal Energy02:00

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The total of all possible kinds of energy present in a substance is called the internal energy (U), sometimes symbolized as E. Suppose a system with initial internal energy, Uinitial, undergoes a change in energy (transfer of work or heat), and the final internal energy of the system is Ufinal. Change in internal energy equals the difference between Ufinal and Uinitial.
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Alternative drug dissolution methods include the rotating bottle, intrinsic dissolution test, peristalsis, and the Franz diffusion cell method. The rotating bottle method involves meticulously rotating tightly capped controlled-release beads in a temperature-controlled bath. Periodic decanting of samples allows for residue assay, followed by refilling with fresh medium and testing at various pH levels to emulate the gastrointestinal tract conditions.In contrast, the intrinsic dissolution test...
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Related Experiment Video

Updated: Feb 2, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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Inverse problem based on the fast alternating direction method of multipliers algorithm in multiangle total internal

Jinyu Li, Wei Han, Yanmin Li

    Applied Optics
    |November 22, 2018
    PubMed
    Summary

    An improved algorithm enhances axial superresolution in multiangle total internal reflection fluorescence microscopy (TIRFM) imaging. This method achieves 40 nm resolution, improving reconstruction speed and quality for 3D biological structures.

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    Area of Science:

    • Biophysics
    • Microscopy
    • Computational Biology

    Background:

    • Multiangle total internal reflection fluorescence microscopy (TIRFM) is crucial for axial superresolution.
    • Solving the inverse problem is key to TIRFM's superresolution capabilities.

    Purpose of the Study:

    • To apply an improved alternating direction method of multipliers (ADMM) algorithm for solving the inverse problem in multiangle TIRFM.
    • To validate the algorithm's accuracy and compare its performance against existing methods.

    Main Methods:

    • Implementation of an improved ADMM algorithm to reconstruct simulated microtubule structures from multiangle TIRFM images.
    • Comparison of reconstruction times and convergence speeds between the improved and original ADMM algorithms.

    Main Results:

    • The improved algorithm achieved an axial resolution of 40 nm.
    • Demonstrated reduced influence of the penalty parameter on convergence and improved iterative process speed.
    • Successfully reconstructed 3D images with depth information of microtubules and mitochondria.

    Conclusions:

    • The enhanced ADMM algorithm significantly improves axial superresolution in multiangle TIRFM.
    • The algorithm offers faster convergence and better image quality for 3D biological imaging.
    • This method provides valuable depth information for cellular structures like microtubules and mitochondria.