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Implementation of a Single Quadrupole Mass Spectrometer for Fingerprint Analysis: Venenum bufonis as a Case Study
Wenlong Wei1,2, Xia Wang3, Jinjun Hou4
1School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 210009, China. 13521032532@163.com.
Molecules (Basel, Switzerland)
|November 23, 2018
Summary
Mass spectrometry fingerprinting (MSF) offers superior quality control for traditional Chinese medicine (TCM). This method reveals more chemical markers than traditional detectors, ensuring reliable TCM product evaluation.
Area of Science:
- Analytical Chemistry
- Pharmacognosy
- Traditional Chinese Medicine (TCM) analysis
Background:
- Mass spectrometry (MS) is utilized for chemical profiling of TCM.
- Quality control of TCM using mass spectrometry fingerprint (MSF) is limited due to complexity and cost.
Purpose of the Study:
- To extend the application of MSF for TCM quality evaluation.
- To establish and validate an MSF method for quality control of Venenum bufonis (VB).
Main Methods:
- Established a mass spectrometry fingerprint (MSF) method using single quadrupole mass spectrometry.
- Validated MSF performance using similarity and principal component analysis (PCA).
- Compared MSF with photo-diode array (PDA) detection.
Main Results:
- MSF provided more comprehensive chemical information and identified more potential chemical markers (eight vs. four) compared to PDA.
- MSF demonstrated high consistency with triple quadrupole (QQQ) and Q-TOF mass spectrometry.
- The MSF method showed robustness with minimal parameter adjustments.
Conclusions:
- Mass spectrometry fingerprinting (MSF) is a superior method for TCM quality control.
- MSF enhances the identification of chemical markers and ensures reliable TCM evaluation.
- The established MSF method is suitable for widespread application in TCM quality control.
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