The Trans Golgi Region is a Labile Intracellular Ca²⁺ Store Sensitive to Emetine

The Golgi apparatus (GA) is a bona fide Ca²⁺ store; however, there is a lack of GA-specific Ca²⁺ mobilizing agents. Here, we report that emetine specifically releases Ca²⁺ from GA in HeLa and HL-1 atrial myocytes. Additionally, it has become evident that the trans-Golgi is a labile Ca²⁺ store that requires a continuous source of Ca²⁺ from either the external milieu or from the ER, to enable it to produce a detectable transient increase in cytosolic Ca²⁺. Our data indicates that the emetine-sensitive Ca²⁺ mobilizing mechanism is different from the two classical Ca²⁺ release mechanisms, i.e. IP₃ and ryanodine receptors. This newly discovered ability of emetine to release Ca²⁺ from the GA may explain why chronic consumption of ipecac syrup has muscle side effects.