Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PCR01:32

PCR

237.4K
Overview
237.4K
RNA Interference01:23

RNA Interference

28.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.1K
Stereotypes, Prejudice, and Discrimination02:55

Stereotypes, Prejudice, and Discrimination

95.4K
Humans are very diverse and although we share many similarities, we also have many differences. The social groups we belong to help form our identities (Tajfel, 1974). These differences may be difficult for some people to reconcile, which may lead to prejudice toward people who are different. Prejudice is a negative attitude and feeling toward an individual based solely on one’s membership in a particular social group (Allport, 1954; Brown, 2010). Prejudice is common against people who...
95.4K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

40.8K
Overview
40.8K
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

5.1K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
5.1K
Interference and Diffraction02:18

Interference and Diffraction

52.4K
Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
52.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The gut microbiota as a potential biomarker in patients with EGFR-mutant lung cancer.

Scientific reports·2025
Same author

Host desmosomal proteins interacting with human parvovirus B19 genomic DNA.

Virus genes·2025
Same author

Novel reporter systems to detect cold and osmotic stress responses.

Biology methods & protocols·2025
Same author

Techniques to detect physical interactions between genomic regions.

Epigenomics·2025
Same author

Utility of circulating human papillomavirus DNA as a biomarker for detection and prognosis of cervical cancer in Japanese patients.

International journal of clinical oncology·2025
Same author

Blocking RPA-based methods for the determination of CpG methylation status and detection of gene mutations.

Epigenomics·2025

Related Experiment Video

Updated: Feb 2, 2026

Author Spotlight: A Precise and Quantifiable Method for Collecting Hemolymph from Small Arthropods
03:39

Author Spotlight: A Precise and Quantifiable Method for Collecting Hemolymph from Small Arthropods

Published on: April 28, 2023

2.6K

A refined two-step oligoribonucleotide interference-PCR method for precise discrimination of nucleotide differences.

Toshitsugu Fujita1,2, Miyuki Yuno2, Fusako Kitaura2

  • 1Department of Biochemistry and Genome Biology, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, 036-8562, Aomori, Japan.

Scientific Reports
|November 23, 2018
PubMed
Summary

We optimized oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) using a two-step cycling method. This enhanced ORNi-PCR precisely detects single-nucleotide differences, even in rare cancer cells.

More Related Videos

A Cognitive Paradigm to Investigate Interference in Working Memory by Distractions and Interruptions
10:38

A Cognitive Paradigm to Investigate Interference in Working Memory by Distractions and Interruptions

Published on: July 16, 2015

14.1K
Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
15:16

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR

Published on: July 22, 2012

17.8K

Related Experiment Videos

Last Updated: Feb 2, 2026

Author Spotlight: A Precise and Quantifiable Method for Collecting Hemolymph from Small Arthropods
03:39

Author Spotlight: A Precise and Quantifiable Method for Collecting Hemolymph from Small Arthropods

Published on: April 28, 2023

2.6K
A Cognitive Paradigm to Investigate Interference in Working Memory by Distractions and Interruptions
10:38

A Cognitive Paradigm to Investigate Interference in Working Memory by Distractions and Interruptions

Published on: July 16, 2015

14.1K
Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
15:16

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR

Published on: July 22, 2012

17.8K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) inhibits PCR amplification to detect sequence variations.
  • Previous ORNi-PCR methods require refinement for precise single-nucleotide difference detection.

Purpose of the Study:

  • To refine the ORNi-PCR method for precise discrimination of single-nucleotide differences.
  • To establish a detailed technical protocol for the optimized ORNi-PCR.

Main Methods:

  • Developed a two-step (denaturing and annealing plus elongation) cycling protocol instead of the standard three-step method.
  • Optimized ORNi-PCR using two-step cycles for stable ORN-DNA hybridization and sequence-specific amplification suppression.
  • Validated the method on genomic DNA and cDNA with induced mutations or genome editing.

Main Results:

  • The optimized Two-Step ORNi-PCR method accurately discriminates single-nucleotide differences in various DNA/cDNA samples.
  • Successfully detected cancer cells with a single nucleotide mutation (0.2% of total cells) within a wild-type population.
  • Demonstrated the method's utility for detecting low-frequency genetic variations.

Conclusions:

  • Two-Step ORNi-PCR offers a simple, precise, and cost-effective method for detecting nucleotide differences.
  • The optimized protocol enhances sequence-specific amplification suppression for reliable mutation detection.
  • This technique has broad applications in molecular biology and medical diagnostics, particularly for sensitive mutation screening.