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Application of Multiple Kits in Special Parentage Testing Cases.

H M Gao1,2, C Wang1,2, S S Zhang1

  • 1Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China.

Fa Yi Xue Za Zhi
|November 23, 2018
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Summary

Genetic analysis of 21 autosome short tandem repeat (STR) loci in the Han population of Shandong Province reveals high polymorphism for paternity identification. Some mutation cases require additional STR loci for accurate typing.

Keywords:
forensic geneticskitparent-child relationspaternity identification

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Area of Science:

  • Forensic genetics
  • Population genetics
  • Human identification

Background:

  • Short tandem repeat (STR) loci are crucial for human identification and paternity testing.
  • Understanding genetic polymorphism in specific populations is essential for accurate forensic analysis.

Purpose of the Study:

  • To analyze the genetic polymorphism of 21 autosome STR loci in the Han population of Shandong Province.
  • To evaluate the performance of the GoldenEye® DNA identification system 25A in cases with STR loci mutation or allelic loss.

Main Methods:

  • Typing of 40 autosome STR loci in 273 unrelated individuals using GoldenEye® DNA identification systems (25A and 22NC).
  • Analysis of genetic polymorphism for 21 STR loci.
  • Investigation of mutation and allelic loss cases using GoldenEye® systems (22NC, 20Y, 17X), AmpFℓSTR® Identifiler® Plus PCR, and gene sequencing.

Main Results:

  • Established genetic parameters for 21 autosome STR loci in the Han population of Shandong Province.
  • Identified five cases with loci mutation meeting identification requirements when using up to 40 STR loci.
  • Gene sequencing revealed base mutations or loss in primer binding domains for allelic loss cases on D18S51; D13S317 allelic loss was confirmed using a different kit.

Conclusions:

  • The 21 autosome STR loci in the Han population of Shandong Province exhibit high polymorphism suitable for routine paternity identification.
  • The GoldenEye® DNA identification system 25A may be insufficient for certain mutation cases, necessitating additional STR loci.
  • Gene sequencing or alternative commercial kits can resolve allelic loss discrepancies.