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Related Experiment Video

Updated: Feb 2, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

[Advances of sequencing and assembling technologies for complex genomes].

Sheng Han Gao¹, Hai Ying Yu¹, Shuang Yang Wu¹

¹CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.

Yi Chuan = Hereditas

|November 23, 2018

Summary

Sequencing complex genomes, often repetitive and heterozygous, requires advanced DNA extraction, sequencing technologies, and assembly algorithms. This review details current strategies for overcoming these genomic challenges.

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Last Updated: Feb 2, 2026

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Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Area of Science:

Genomics
Bioinformatics

Background:

Complex genomes present significant challenges for standard sequencing and assembly methods.
Characteristics include high repetitiveness, heterozygosity, GC bias, and contamination.

Purpose of the Study:

To review state-of-the-art technologies and strategies for sequencing and assembling complex genomes.
To address challenges in complex genome research.

Main Methods:

Summarizing advancements in DNA extraction techniques.
Reviewing modern sequencing technologies and platforms.
Analyzing algorithms and strategies for genome assembly.

Main Results:

Identification of key techniques in DNA extraction, sequencing, and assembly.
Overview of representative complex genome sequencing projects.
Discussion of technological developments for complex genome challenges.

Conclusions:

Effective sequencing and assembly of complex genomes rely on integrated approaches.
Continued development in DNA extraction, sequencing, and assembly is crucial for advancing genomic research.