Related Experiment Video
Updated: Feb 2, 2026
15:19
Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
10.1K
Paracandidimonas caeni sp. nov., isolated from sludge
1College of Marine and Bio-Engineering, Yancheng Teachers University, Yancheng, Jiangsu 210095, PR China.
International Journal of Systematic and Evolutionary Microbiology
|November 23, 2018
Summary
A novel bacterium, Paracandidimonas caeni 24T, was discovered in pesticide factory sludge. This beige-pigmented, Gram-negative rod represents a new species within the Paracandidimonas genus.
Area of Science:
- Microbiology
- Bacteriology
- Environmental Science
Background:
- Microbial communities in industrial sludge can possess unique metabolic capabilities.
- Identification and characterization of novel bacteria are crucial for understanding biodiversity and potential applications.
Purpose of the Study:
- To isolate and characterize a novel bacterial strain from pesticide manufacturing factory sludge.
- To determine the taxonomic affiliation of the novel isolate using polyphasic characterization.
Main Methods:
- Isolation and cultivation of bacteria from sludge.
- 16S rRNA gene sequencing for phylogenetic analysis.
- DNA-DNA hybridization for genetic relatedness assessment.
- Analysis of cellular fatty acids, respiratory quinones, polyamines, and polar lipids.
- Determination of G+C content.
Main Results:
- A beige-pigmented, Gram-negative, aerobic, non-motile rod, strain 24T, was isolated.
- 16S rRNA gene sequence analysis showed highest similarity to Parapusillimonas granuli Ch07T (98.20%).
- Phylogenetic analysis and DNA-DNA hybridization (<45%) confirmed strain 24T as a distinct species within the genus Paracandidimonas.
- Chemotaxonomic analyses revealed Q-8, putrescine, specific fatty acids, and polar lipids consistent with the genus.
Conclusions:
- Strain 24T represents a novel species of the genus Paracandidimonas.
- The proposed name for this new species is Paracandidimonas caeni sp. nov.
- The type strain is 24T (=CCTCC AB 2018057T=KACC 19692T).
More Related Videos
Related Concept Videos
01:24DNA Isolation
45.1K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
45.1K
01:34DNA Isolation
199.5K
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
199.5K
01:20Overview Of Cell Separation And Isolation
7.6K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
7.6K
01:19Cyclic Processes And Isolated Systems
3.5K
A thermodynamic system with zero heat exchange and work is an isolated system. For these systems, the internal energy remains constant.
In the case of a non-isolated system, the change in the internal energy is zero only if the process is cyclic. A thermodynamic process is considered cyclic if the system undergoes a series of changes and returns to its initial state.
Consider a cyclic process that returns to its initial state, undergoing a four-step process. The heat transfer along each...
In the case of a non-isolated system, the change in the internal energy is zero only if the process is cyclic. A thermodynamic process is considered cyclic if the system undergoes a series of changes and returns to its initial state.
Consider a cyclic process that returns to its initial state, undergoing a four-step process. The heat transfer along each...
3.5K
01:24Techniques for Isolation of Pure Cultures
2.4K
Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...
2.4K
01:12Reduced Mass Coordinates: Isolated Two-body Problem
2.4K
In classical mechanics, the two-body problem is one of the fundamental problems describing the motion of two interacting bodies under gravity or any other central force. When considering the motion of two bodies, one of the most important concepts is the reduced mass coordinates, a quantity that allows the two-body problem to be solved like a single-body problem. In these circumstances, it is assumed that a single body with reduced mass revolves around another body fixed in a position with an...
2.4K