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A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications
Published on: September 16, 2022
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Liquid biopsy: novel technologies and clinical applications
Natalie Reimers1, Klaus Pantel2
1Department of Tumor Biology, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany.
Clinical Chemistry and Laboratory Medicine
|November 23, 2018
Summary
Liquid biopsy, a novel diagnostic approach introduced in 2010, analyzes circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) in cancer patients. Over the last decade, diverse methods have emerged for detecting these biomarkers in peripheral blood.
Area of Science:
- Oncology
- Molecular Diagnostics
- Biochemistry
Background:
- Liquid biopsy emerged in 2010 as a diagnostic concept.
- Initial focus was on circulating tumor cells (CTCs).
- The concept has expanded to include circulating tumor DNA (ctDNA).
Purpose of the Study:
- To review the evolution of liquid biopsy.
- To highlight the development of detection methods for CTCs and ctDNA.
- To underscore the significance of liquid biopsy in cancer diagnostics.
Main Methods:
- Analysis of circulating tumor cells (CTCs).
- Detection of circulating tumor DNA (ctDNA) in peripheral blood.
- Review of various established and emerging detection methodologies.
Main Results:
- Liquid biopsy has broadened its scope beyond CTCs to include ctDNA.
- Significant advancements in detection techniques have been made over the past decade.
- These methods enable non-invasive monitoring of cancer patients.
Conclusions:
- Liquid biopsy represents a rapidly advancing field in cancer diagnostics.
- The development of sensitive detection methods is crucial for clinical application.
- Liquid biopsy holds great promise for personalized cancer management.
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