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Pectic oligosaccharides hydrolyzed from citrus canning processing water by Fenton reaction and their
Junhui Li1, Shan Li1, Shanshan Liu1
1Zhejiang Key Laboratory for Agro-Food Processing, Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China.
Abstract:
Citrus canning processing water contains a valuable and renewable source of biopolymers and bioactive compounds including pectic polysaccharides. Upgrading these processing wastes can not only alleviate environmental pollution but also add value to the commodity's production. In a previous study we recovered pectic polysaccharides from citrus canning processing water. In the present study, pectic polysaccharides recycled from citrus canning processing water was depolymerized by an optimized Fenton system. The hydrolyzate was fractionated via size-exclusion chromatography into six fractions: 500 Da < LMP1 < 3 kDa; 3 kDa < LMP2 < 5 kDa; 5 kDa < LMP3 < 12 kDa; 12 kDa < LMP4 < 25 kDa; 25 kDa < LMP5 < 100 kDa and LMP6 > 10 wDa. Structure analyses showed that LMP1 were homogalacturonans-enriched non-esterified polysaccharides. While LMP2 contained both HG and rhamnogalacturonan-I (RG-I). Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition.
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