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Bone marrow mesenchymal stem cells attenuate the progression of focal segmental glomerulosclerosis in rat models

Ru-Chun Yang1, Xiao-Ling Zhu2, Jun Wang1

  • 1Departmgent of Nephrology (Key laboratory of Zhejiang province, management of kidney disease), Hangzhou Hospital of Traditional Chinese Medicine, Tiyuchang Road 453, Hangzhou, 310007, People's Republic of China.

BMC Nephrology
|November 24, 2018
PubMed
Abstract

Insights

Bone marrow mesenchymal stem cells (BMSCs) show protective effects in a rat model of focal segmental glomerulosclerosis (FSGS). BMSC transplantation reduced kidney damage, improving outcomes for FSGS progression.

Area of Science:

  • Nephrology
  • Regenerative Medicine
  • Stem Cell Biology

Background:

  • Focal segmental glomerulosclerosis (FSGS) is a leading cause of end-stage kidney disease.
  • Mesenchymal stem cells (MSCs) demonstrate reparative potential in kidney injury.
  • The specific impact of bone marrow mesenchymal stem cells (BMSCs) on FSGS progression requires further elucidation.

Purpose of the Study:

  • To investigate the protective effects of BMSCs in a rat model of FSGS.
  • To determine if BMSC transplantation can attenuate the progression of kidney damage in FSGS.

Main Methods:

  • A rat model of FSGS was established using unilateral nephrectomy and adriamycin injection.
  • Rat BMSCs were isolated, characterized, and transplanted into FSGS recipients.
  • Kidney function (proteinuria, creatinine, urea nitrogen), renal morphology, and molecular markers (MMP9, TIMP-1, IL-6, TNF-α) were assessed post-transplantation.

Main Results:

  • BMSC transplantation significantly reduced proteinuria, serum creatinine, and urea nitrogen levels.
  • Histological analysis revealed amelioration of renal morphology in BMSC-treated rats.
  • BMSC transplantation modulated the MMP9/TIMP-1 ratio and downregulated proinflammatory cytokines (IL-6, TNF-α).

Conclusions:

  • BMSC transplantation effectively attenuates FSGS progression in a preclinical rat model.
  • These findings provide a theoretical basis for using autologous BMSCs in clinical FSGS therapy.
Keywords:
BMSCFSGSMMP9/TIMP-1Proinflammatory cytokinesRat

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