Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Accelerators01:17

Accelerators

288
Accelerators in concrete serve as admixtures to speed up the hardening process, enabling the concrete to achieve early strength faster. Although accelerators do not necessarily impact the time it takes concrete to set, they reduce this time in practice. A common accelerator is calcium chloride, which is particularly useful for hastening early strength development in cold weather or for rapid repair jobs that require quick heat generation after mixing.
The effectiveness of calcium chloride can...
288
Average Acceleration01:30

Average Acceleration

13.7K
The importance of understanding acceleration spans our day-to-day experiences, as well as the vast reaches of outer space and the tiny world of subatomic physics. In everyday conversation, to accelerate means to speed up. For instance, we are familiar with the acceleration of our car; the harder we apply our foot to the gas pedal, the faster we accelerate. The greater the acceleration, the greater the change in velocity over a given time. Acceleration is widely seen in experimental physics. In...
13.7K
Instantaneous Acceleration01:16

Instantaneous Acceleration

23.1K
Acceleration is in the direction of the change in velocity, but it is not always in the direction of motion. When an object slows down, its acceleration is opposite to the direction of its motion. Although commonly referred to as deceleration, this causes confusion in our analysis as deceleration is not a vector, and does not point to a specific direction with respect to a coordinate system. Therefore, the term deceleration is not used. For example, when a subway train slows down, it...
23.1K
Acceleration Vectors01:30

Acceleration Vectors

22.6K
In everyday conversation, accelerating means speeding up. Acceleration is a vector in the same direction as the change in velocity, Δv, therefore the greater the acceleration, the greater the change in velocity over a given time. Since velocity is a vector, it can change in magnitude, direction, or both. Thus acceleration is a change in speed or direction, or both. For example, if a runner traveling at 10 km/h due east slows to a stop, reverses direction, and continues their run at 10 km/h...
22.6K
Accelerating Fluids01:17

Accelerating Fluids

2.3K
When a fluid is in constant acceleration, the pressure and buoyant force equations are modified. Suppose a beaker is placed in an elevator accelerating upward with a constant acceleration, a. In the beaker, assume there is a thin cylinder of height h with an infinitesimal cross-sectional area, ΔS.
The motion of the liquid within this infinitesimal cylinder is considered to obtain the pressure difference. Three vertical forces act on this liquid:
2.3K
Acceleration due to Gravity on Other Planets01:24

Acceleration due to Gravity on Other Planets

5.0K
The gravitational acceleration of an object near the Earth's surface is called the acceleration due to gravity. It can be measured by conducting simple experiments on Earth. However, such an experiment is impossible to conduct on the surface of other planets.
Astronomical observations are thus used to measure the acceleration due to gravity on other planets. This can be determined by observing the effect of a planet's gravity on objects close to it. The crucial factor that helps in this...
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human RNA polymerase III termination favors decomposition over facilitated recycling.

Nature communications·2026
Same author

Functional Ureteral Obstruction Due to Retroperitoneal Tissue Interposition During Oblique Lumbar Interbody Fusion: A Report of Two Cases.

Journal of clinical medicine·2026
Same author

Asymptomatic tuberculosis detected in health screening predicts favourable outcome.

ERJ open research·2026
Same author

8-λ LAN-WDM TOSA for 800-Gb/s links with simplified, non-hermetic packaging.

Optics express·2026
Same author

High prevalence of persistent tuberculosis-related symptoms 6 months after treatment in pulmonary tuberculosis.

BMJ open respiratory research·2026
Same author

Radiomics identifies distinct cortical bone texture alterations in patients with CKD using HR-pQCT.

Bone research·2026
Same journal

Genetically predicted CXCL16 expression is associated with Parkinson's disease risk and peripheral immune cell dysregulation: a two-sample mendelian randomization study.

Molecular brain·2026
Same journal

Endovascular stem cell therapy reconfigures post-stroke ER dynamics via GRP78/Atlastin/CHOP axis.

Molecular brain·2026
Same journal

OptoH<sub>3</sub>R fusion protein mimics β-arrestin-mediated membrane endocytosis of histamine H<sub>3</sub> receptor in vitro.

Molecular brain·2026
Same journal

Generating models for isoform-specific PKM-KIBRA interactions with BIFC, stabilization and AlphaFold 3.

Molecular brain·2026
Same journal

Therapeutic potential of AdipoRon in cognitive, depressive, and anxiety disorders: a systematic review and meta-analysis.

Molecular brain·2026
Same journal

PAK1 expression protects cellular and behavioral defects in animal models of Parkinson' s disease.

Molecular brain·2026
See all related articles

Related Experiment Video

Updated: Feb 2, 2026

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
19:05

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Published on: October 30, 2015

12.9K

Accelerated FRET-PAINT microscopy.

Jongjin Lee1, Sangjun Park1, Sungchul Hohng2,3

  • 1Department of Physics and Astronomy, Seoul National University, Seoul, 08826, Republic of Korea.

Molecular Brain
|November 24, 2018
PubMed
Summary
This summary is machine-generated.

Researchers optimized FRET-PAINT microscopy for faster super-resolution imaging. This enhanced technique achieves high-quality 40-nm resolution images of microtubules in seconds, overcoming previous speed limitations.

Keywords:
FRETFRET-PAINTSingle-molecule localization microscopySuper-resolution fluorescence microscopy

More Related Videos

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy
10:59

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Published on: October 18, 2024

1.3K
FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
10:34

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Published on: August 20, 2012

23.7K

Related Experiment Videos

Last Updated: Feb 2, 2026

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
19:05

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Published on: October 30, 2015

12.9K
Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy
10:59

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Published on: October 18, 2024

1.3K
FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
10:34

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Published on: August 20, 2012

23.7K

Area of Science:

  • Biophysics
  • Microscopy
  • Molecular Imaging

Background:

  • DNA-PAINT is a super-resolution microscopy technique known for its resistance to photobleaching.
  • Förster Resonance Energy Transfer (FRET)-PAINT microscopy has recently enhanced DNA-PAINT's imaging speed.
  • Further optimization is needed to reach the theoretical speed limits of FRET-PAINT.

Purpose of the Study:

  • To achieve the ultimate speed limit of FRET-PAINT microscopy.
  • To increase the imaging speed of FRET-PAINT by an additional 8-fold.
  • To obtain high-quality super-resolution images in a significantly reduced timeframe.

Main Methods:

  • Optimizing camera speed for faster data acquisition.
  • Adjusting the dissociation rate of DNA probes to control blinking dynamics.
  • Minimizing bleed-through of the donor signal to the acceptor channel.
  • Applying these optimizations to FRET-PAINT microscopy.

Main Results:

  • Achieved an 8-fold increase in FRET-PAINT imaging speed.
  • Obtained high-quality super-resolution images with 40-nm resolution.
  • Successfully imaged COS-7 cell microtubules in tens of seconds.

Conclusions:

  • The optimized FRET-PAINT microscopy pushes the boundaries of super-resolution imaging speed.
  • High-resolution imaging of biological structures is now achievable in seconds.
  • This advancement holds potential for dynamic biological process studies at the nanoscale.