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Long non-coding RNAs in hematological malignancies: translating basic techniques into diagnostic and therapeutic
Nonthaphat Kent Wong1, Chien-Ling Huang2, Rashidul Islam1
1Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Y9/F, Lee Shau Kee Building, Hung Hom, Hong Kong SAR, China.
Journal of Hematology & Oncology
|November 24, 2018
Summary
Long non-coding RNAs (lncRNAs) play roles in diseases like leukemia. This review discusses methods to study lncRNAs, including CRISPR-Cas gene editing, for potential biomarkers and therapies.
Area of Science:
- Genomics
- Molecular Biology
- Biochemistry
Background:
- The human genome contains vast non-coding regions, including diverse long non-coding RNAs (lncRNAs).
- Abnormal lncRNA expression is linked to diseases such as cancers, but their functions and regulation remain unclear.
- lncRNAs present unique challenges for study due to functional diversity and low cross-species conservation.
Purpose of the Study:
- To review methods for identifying and functionally characterizing lncRNAs in hematological malignancies.
- To highlight the potential of advanced tools like the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system for lncRNA research.
- To discuss the role of lncRNAs in leukemia pathogenesis and progression.
Main Methods:
- Review of conventional and advanced techniques for lncRNA identification and functional analysis.
- Discussion of CRISPR-Cas gene editing strategies for lncRNA manipulation (knock-in/out).
- Analysis of lncRNA roles in leukemia development and progression.
Main Results:
- lncRNAs are implicated in various biological processes and human diseases, including hematological malignancies.
- CRISPR-Cas systems offer powerful tools for dissecting lncRNA functions.
- Understanding lncRNA roles in leukemia can inform biomarker and therapeutic development.
Conclusions:
- lncRNAs are critical players in hematological malignancies, particularly leukemia.
- Advanced techniques, especially CRISPR-Cas, are revolutionizing lncRNA research.
- Further investigation into lncRNAs may yield novel clinical applications for cancer treatment.
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