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Solid-phase enzymoimmunoassay of estrone in serum
J Folan1, J P Gosling, P F Fottrell
1Department of Biochemistry, University College, Galway, Ireland.
Clinical Chemistry
|September 1, 1988
Summary
A new solid-phase enzymoimmunoassay offers a rapid and convenient method for measuring estrone (a key estrogen) in serum. This assay provides accurate results comparable to radioimmunoassays, simplifying clinical diagnostics.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Endocrinology
Background:
- Estrone is a significant estrogen hormone.
- Accurate measurement of estrone is crucial for various clinical applications.
- Existing radioimmunoassays for estrone can be complex and time-consuming.
Purpose of the Study:
- To develop and validate a rapid, solid-phase enzymoimmunoassay for estrone in serum or plasma.
- To assess the assay's sensitivity, precision, and correlation with established methods.
- To evaluate the assay's convenience for routine clinical use.
Main Methods:
- Solid-phase enzymoimmunoassay performed on microtiter plates.
- Serum samples extracted with diethyl ether prior to analysis.
- No chromatographic or centrifugation steps required.
- Detection limit established at 380 fg per well (28 pmol/L).
Main Results:
- High precision demonstrated with low intra- and interassay coefficients of variation (e.g., 2.0% and 6.3% for high concentrations).
- Excellent correlation (r=0.95) with a commercial radioimmunoassay.
- Assay is simple to perform, allowing one person to process 80 samples daily.
Conclusions:
- The developed enzymoimmunoassay is highly suitable for serum estrone measurements.
- The assay offers greater convenience and simplicity compared to existing radioimmunoassays.
- This method provides a valuable tool for efficient clinical estrone analysis.