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Updated: Feb 2, 2026
11:22
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
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Progress in the 16S rRNA Gene Sequencing in Forensic Science
G Q Song1,2,3, Y Cao2,3, H Li2,3
1Department of Forensic Medicine, School of Basic Medical Science, Fudan University, Shanghai 200032, China.
Fa Yi Xue Za Zhi
|November 24, 2018
Summary
Forensic microbiology utilizes 16S rRNA gene sequencing for identifying biological samples, estimating time since death, and determining geographic origins. This method enhances traditional forensic techniques with high-throughput sequencing technology.
Area of Science:
- Forensic Science
- Microbiology
- Genetics
Background:
- Forensic microbiology is a key area in forensic science research.
- The 16S rRNA gene is a conserved and specific marker ideal for forensic identification.
- High-throughput sequencing technology enables advanced microbial research applicable to forensic science.
Purpose of the Study:
- To describe research methods and sequencing technologies for 16S rRNA gene sequencing.
- To summarize the research progress of 16S rRNA gene sequencing in forensic science.
- To discuss the application value and future potential of 16S rRNA in forensic investigations.
Main Methods:
- 16S rRNA gene sequencing techniques.
- High-throughput sequencing technologies.
- Analysis of microbial DNA for forensic applications.
Main Results:
- 16S rRNA gene sequencing is applied to forensic practice.
- Applications include biological sample identification, individual identification, postmortem interval estimation, and regional inference.
- Forensic microbiology complements and assists traditional forensic methods.
Conclusions:
- 16S rRNA gene sequencing offers significant value in forensic science.
- Its application provides crucial clues for case investigations.
- Continued research will expand its potential in forensic practice.
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