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Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library
Published on: September 30, 2019
Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA
Neeraj N Patwardhan1, Zhengguo Cai, Colby N Newson
1Department of Chemistry, 124 Science Drive, Box 90346, Durham, NC 27708, USA. amanda.hargrove@duke.edu.
Abstract:
A prominent hurdle in developing small molecule probes against RNA is the relative scarcity of general screening methods. In this study, we demonstrate the application of a fluorescent peptide displacement assay to screen small molecule probes against four different RNA targets. The designed experimental protocol combined with statistical analysis provides a fast and convenient method to simultaneously evaluate small molecule libraries against different RNA targets and classify them based on affinity and selectivity patterns.
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