Hydrogel Microelectrodes for the Rapid, Reliable, and Repeatable Characterization of Lipid Membranes

Model lipid bilayer membranes provide approximations of natural cellular membranes that may be formed in the laboratory to study their mechanics and interactions with the surrounding environment. A new approach for their formation is proposed here based on the self-assembly of lipid monolayers at oil-water interfaces, creating a lipid-coated hydrogel-tipped electrode that produces a stable lipid membrane on the surface when introduced to a lipid-coated aqueous droplet. Membrane formation using the hydrogel microelectrode is tested for a variety of lipids and oils. The channel-forming peptide alamethicin is added to the membrane, and its functionality is verified. Finally, asymmetric membranes are created using varying lipid compositions, and the capacity for repeated quantification of membrane structure is demonstrated. The proposed hydrogel microelectrodes are compatible with multiple oils and lipids, simple to use, and suitable for detecting the presence of both biomolecular transporters and dissolved lipid compositions within aqueous droplets.