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Expression of nuclear lamins during mouse preimplantation development
E Houliston1, M N Guilly, J C Courvalin
1Centre de Génétique Moléculaire CNRS, Gif sur Yvette, France.
Development (Cambridge, England)
|February 1, 1988
Summary
Nuclear lamins are present throughout mouse preimplantation development. Their synthesis indicates lamins are produced early, suggesting a role in cell differentiation.
Area of Science:
- Cell Biology
- Developmental Biology
- Molecular Biology
Background:
- Nuclear lamins are essential structural proteins of the nuclear envelope.
- Their role during early mammalian development is not fully understood.
Purpose of the Study:
- To investigate the expression and synthesis of nuclear lamins during mouse preimplantation development.
- To explore the potential role of lamins in early cell differentiation.
Main Methods:
- Immunofluorescence staining of nuclear lamins in mouse embryos.
- Immunoblotting to detect lamin presence and apparent molecular weight.
- Immunoprecipitation to assess the synthesis of specific lamins.
Main Results:
- Nuclear lamins (A, B, and C) are present from the fertilized egg to the blastocyst stage.
- Lamin A in eggs shows a higher apparent molecular weight than in later stages.
- Synthesis of lamin A is detected in eggs, while lamin B synthesis begins by the 8-cell stage.
Conclusions:
- Nuclear lamins are expressed and synthesized early in mouse development.
- The observed synthesis suggests lamins are not solely maternally provided.
- These findings support a potential role for nuclear lamins in early cell differentiation.