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Related Experiment Video

Updated: Feb 2, 2026

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM

Published on: November 1, 2017

Integrated digital holographic microscopy based on surface plasmon resonance.

Siqing Dai, Jiwei Zhang, Hua Lu

|November 25, 2018

Summary

This study introduces an integrated digital holographic microscopy (DHM) combining surface plasmon holographic microscopy (SPHM) and reflection DHM. This novel technique accurately measures both refractive index and thickness variations in specimens.

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Last Updated: Feb 2, 2026

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM

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Engineering Antiviral Agents via Surface Plasmon Resonance

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Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

Area of Science:

Optics and Photonics
Materials Science
Biophysics

Background:

Digital holographic microscopy (DHM) offers non-invasive imaging capabilities.
Surface plasmon resonance (SPR) provides high sensitivity for detecting minute changes in refractive index.
Reflection DHM is effective for measuring specimen thickness variations.

Purpose of the Study:

To develop a novel integrated digital holographic microscopy (DHM) system.
To combine the strengths of surface plasmon holographic microscopy (SPHM) and reflection DHM.
To enable simultaneous measurement of refractive index and thickness changes in specimens.

Main Methods:

Integration of SPHM and reflection DHM using angular and polarization multiplexing.
Leveraging the high sensitivity of SPR and the high reflectivity of gold films.
Experimental monitoring of an alcohol-water mixture droplet volatilization process.

Main Results:

Demonstrated simultaneous measurement of refractive index and thickness variations.
Successfully monitored the dynamic volatilization process of a liquid mixture.
Verified the effectiveness of the integrated DHM system for low-contrast specimens.

Conclusions:

The proposed integrated DHM is a powerful tool for multi-information measurements.
This technique is promising for objective-coupling SPR microscopy.
It enables dynamic and non-destructive analysis of diverse specimens, including biomolecules and nanofluids.