Multi-wavelength multi-angle reflection tomography

Area of Science:

• Optics and Photonics
• Microscopy
• Computational Imaging

Background:

• Traditional microscopy techniques face limitations in resolving complex 3D structures.
• Holography offers potential for 3D information but requires advanced reconstruction methods.
• Reflection tomography provides a pathway for non-invasive 3D imaging.

Purpose of the Study:

• To develop and validate a novel reflection tomographic microscope.
• To implement an iterative inversion algorithm for accurate sample reconstruction.
• To investigate the impact of multiple wavelengths on reconstruction quality.

Main Methods:

• Development of a reflection tomographic microscope system.
• Recording of holograms at various incidence angles and wavelengths.
• Application of an iterative inversion algorithm modeling wave-sample interaction.
• Simultaneous processing of all recorded data for permittivity distribution estimation.

Main Results:

• Successful reconstruction of sample permittivity distribution.
• Demonstration of significant improvement in reconstruction quality using multiple wavelengths.
• Enhanced resolution and accuracy along the optical axis attributed to multi-wavelength approach.

Conclusions:

• The developed reflection tomographic microscope enables high-fidelity 3D imaging.
• Multi-wavelength data acquisition is crucial for superior tomographic reconstruction.
• The iterative inversion algorithm effectively processes complex holographic data for accurate material property mapping.