Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

$Interference and Diffraction$ 02:18

Interference and Diffraction

Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...

Limiting Reactant

Limiting Reactant

The relative amounts of reactants and products represented in a balanced chemical equation are often referred to as stoichiometric amounts. However, in reality, the reactants are not always present in the stoichiometric amounts indicated by the balanced equation.

The Number e as a Limit

The Number e as a Limit

The number e is a fundamental constant in calculus, playing a central role in describing continuous change, particularly exponential growth. It is most naturally defined through its relationship with the natural logarithm, which is the inverse of the exponential function with base e. This relationship allows e to be characterized using basic principles of differentiation rather than as an arbitrary numerical constant.A key property of the natural logarithm function, ln x, is that its derivative...

Reflection of Waves

Reflection of Waves

When a wave travels from one medium to another, it gets reflected at the boundary of the second medium. A common example of this is when a person yells at a distance from a cliff and hears the echo of their voice. The sound waves (longitudinal waves) traveling in the air are reflected from the bounding cliff. Similarly, flipping one end of a string whose other end is tied to a wall causes a pulse (transverse wave) to travel through the string, which gets reflected upon reaching the wall. In...

$X-ray Diffraction of Biological Samples$ 01:10

X-ray Diffraction of Biological Samples

X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Method for label-free & non-destructive detection of microplastics in human formalin-fixed paraffin-embedded tissue sections.

Scientific reports·2025

Same author

Detection of Unlabeled Polystyrene Micro- and Nanoplastics in Mammalian Tissue by Optical Photothermal Infrared Spectroscopy.

Analytical chemistry·2025

Same author

Curing reaction kinetics of paper-based phenolic resin laminates-from laboratory measurements to inline quality control.

Analytical and bioanalytical chemistry·2025

Same author

Mid-infrared hyperspectral single-pixel microscopy with a quantum cascade laser.

Optics express·2025

Same author

Quantum Fourier Transform Infrared Spectroscopy: Evaluation, Benchmarking, and Prospects.

Applied spectroscopy·2025

Same author

All-mirror wavefront division interferometer for Fourier transform spectrometry across multiple spectral ranges.

Optics express·2025

Search research articles

Related Experiment Video

Updated: Feb 2, 2026

In-situ Tapering of Chalcogenide Fiber for Mid-infrared Supercontinuum Generation

In-situ Tapering of Chalcogenide Fiber for Mid-infrared Supercontinuum Generation

Published on: May 27, 2013

Diffraction limited mid-infrared reflectance microspectroscopy with a supercontinuum laser.

Jakob Kilgus, Gregor Langer, Kristina Duswald

|November 25, 2018

Summary

This study demonstrates a new mid-infrared (MIR) microspectroscopy method using a supercontinuum (SC) source for enhanced chemical mapping. The SC source offers superior signal-to-noise, resolution, and speed compared to traditional methods, enabling detailed imaging of polymers and red blood cells.

More Related Videos

$Femtosecond Laser Filaments for Use in Sub-Diffraction-Limited Imaging and Remote Sensing$

Femtosecond Laser Filaments for Use in Sub-Diffraction-Limited Imaging and Remote Sensing

Published on: April 25, 2019

Characterizing Far-infrared Laser Emissions and the Measurement of Their Frequencies

Characterizing Far-infrared Laser Emissions and the Measurement of Their Frequencies

Published on: December 18, 2015

Related Experiment Videos

Last Updated: Feb 2, 2026

In-situ Tapering of Chalcogenide Fiber for Mid-infrared Supercontinuum Generation

In-situ Tapering of Chalcogenide Fiber for Mid-infrared Supercontinuum Generation

Published on: May 27, 2013

$Femtosecond Laser Filaments for Use in Sub-Diffraction-Limited Imaging and Remote Sensing$

Femtosecond Laser Filaments for Use in Sub-Diffraction-Limited Imaging and Remote Sensing

Published on: April 25, 2019

Characterizing Far-infrared Laser Emissions and the Measurement of Their Frequencies

Characterizing Far-infrared Laser Emissions and the Measurement of Their Frequencies

Published on: December 18, 2015

Area of Science:

Spectroscopy
Microscopy
Materials Science

Background:

Mid-infrared (MIR) spectroscopy is crucial for chemical analysis.
Conventional Fourier-transform infrared (FTIR) microscopy has limitations in speed and resolution.
Supercontinuum (SC) sources offer unique properties for advanced optical techniques.

Purpose of the Study:

To demonstrate a novel MIR microspectroscopy setup utilizing an SC source.
To evaluate the performance of the SC-based system for chemical mapping and imaging.
To compare the SC system's capabilities against conventional FTIR microscopy.

Main Methods:

Development of a MIR microspectroscopy system with an SC source (1.55–4.5 µm) and a MEMS Fabry-Pérot filter spectrometer.
Achieved diffraction-limited spatial resolution in reflection geometry.
Acquisition and analysis of spectral data from multilayer polymer films and red blood cells.

Main Results:

The SC source provided higher signal-to-noise ratios compared to thermal sources.
Improved spatial resolution and faster scanning were achieved with the SC system.
Demonstrated diffraction-limited MIR imaging of red blood cells in reflection mode for the first time.

Conclusions:

The SC-based MIR microspectroscopy system significantly enhances chemical mapping capabilities.
The high brightness, spatial coherence, and broadband nature of SC radiation are beneficial for MIR microscopy.
This technology holds substantial potential for advancing infrared microscopy applications.