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Immunolabeling of lipopolysaccharide liberated from antibiotic-treated Escherichia coli
P M Flynn1, J L Shenep, F Gigliotti
1Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.
Infection and Immunity
|October 1, 1988
Abstract:
Increased anti-core glycolipid antibody binding was visualized by immunoelectron microscopy after incubation of Escherichia coli K1:O7 cells with a bacteriolytic antibiotic and compared with binding in control cells. The findings suggest that the core glycolipid regions of the lipopolysaccharides of some gram-negative bacilli can be effectively sequestered until liberated by antibiotic-induced cell lysis.