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Updated: Feb 2, 2026
Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
TSPYL5-mediated inhibition of p53 promotes human endothelial cell function
Hee-Jun Na1, Chung Eun Yeum1, Han-Seop Kim1
1Stem Cell Research Laboratory, Immunotherapy Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Taejon, 34141, Republic of Korea.
Abstract:
Testis-specific protein, Y-encoded like (TSPYL) family proteins (TSPYL1-6), which are members of the nucleosome assembly protein superfamily, have been determined to be involved in the regulation of various cellular functions. However, the potential role of TSPYL family proteins in endothelial cells (ECs) has not been determined. Here, we demonstrated that the expression of TSPYL5 is highly enriched in human ECs such as human umbilical vein endothelial cells (HUVECs) and human pluripotent stem cell-differentiated ECs (hPSC-ECs). Importantly, TSPYL5 overexpression was shown to promote EC proliferation and functions, such as migration and tube formation, by downregulating p53 expression. Adriamycin-induced senescence was markedly blocked by TSPYL5 overexpression. In addition, the TSPYL5 depletion-mediated loss of EC functions was blocked by p53 inhibition. Significantly, TSPYL5 overexpression promoted angiogenesis in Matrigel plug and wound repair in a mouse skin wound healing model in vivo. Our results suggest that TSPYL5, a novel angiogenic regulator, plays a key role in maintaining endothelial integrity and function. These findings extend the understanding of TSPYL5-dependent mechanisms underlying the regulation of p53-related functions in ECs.
Insights
Testis-specific protein Y-encoded like 5 (TSPYL5) promotes endothelial cell functions and angiogenesis by downregulating p53. TSPYL5 is a novel regulator of endothelial integrity and wound healing.
Area of Science:
- Endocrinology
- Cell Biology
- Molecular Biology
Background:
- Testis-specific protein, Y-encoded like (TSPYL) family proteins regulate cellular functions.
- The role of TSPYL proteins in endothelial cells (ECs) remains unexplored.
Purpose of the Study:
- To investigate the function of TSPYL5 in human endothelial cells.
- To elucidate the role of TSPYL5 in angiogenesis and endothelial integrity.
Main Methods:
- TSPYL5 expression analysis in human ECs (HUVECs, hPSC-ECs).
- Overexpression and depletion of TSPYL5 in ECs.
- Assessment of EC proliferation, migration, and tube formation.
- In vivo angiogenesis (Matrigel plug) and wound healing models in mice.
- Analysis of p53 expression and inhibition.
Main Results:
- TSPYL5 is highly expressed in human ECs.
- TSPYL5 overexpression enhances EC proliferation, migration, and tube formation by downregulating p53.
- TSPYL5 overexpression blocks adriamycin-induced senescence.
- TSPYL5 depletion-induced loss of EC function is rescued by p53 inhibition.
- TSPYL5 promotes angiogenesis and wound repair in vivo.
Conclusions:
- TSPYL5 is a novel angiogenic regulator crucial for endothelial integrity and function.
- TSPYL5 exerts its effects by modulating p53-related pathways in ECs.
- Findings expand understanding of TSPYL5-dependent mechanisms in EC biology.
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