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Surface-tailored anti-HER2/neu-solid lipid nanoparticles for site-specific targeting MCF-7 and BT-474 breast cancer

Eliana B Souto1, Slavomira Doktorovova2, Joana R Campos2

  • 1Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra (FFUC), Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal; CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar 4710-057 Braga, Portugal.

European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences
|November 26, 2018
PubMed

Insights

Antibody-linked solid lipid nanoparticles (SLN) targeting HER2 receptors enhanced cancer cell uptake. This novel approach shows promise for targeted breast cancer therapy, with increased internalization in HER2-positive cells.

Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Oncology

Background:

  • Solid Lipid Nanoparticles (SLN) are investigated for drug delivery.
  • Targeting cancer cells with specific antibodies can improve therapeutic efficacy.
  • Human Epidermal Growth Factor Receptor 2 (HER2) is a key target in breast cancer.

Purpose of the Study:

  • To develop and evaluate antibody-conjugated SLN for targeted delivery to HER2-expressing breast cancer cells.
  • To optimize the conjugation of a compact antibody (CAB51) to SLN via streptavidin-biotin interaction.
  • To assess the cellular uptake and cytotoxic effects of these targeted nanoparticles.

Main Methods:

  • Conjugation of CAB51 antibody to cationic SLN using streptavidin-biotin technology.
  • Optimization of streptavidin and biotinylated antibody loading by analyzing particle size, polydispersity, and stability.
  • Evaluation of cellular uptake and viability in HER2-positive (BT-474) and HER2-negative (MCF-7) breast cancer cell lines.

Main Results:

  • Streptavidin adsorption alone did not affect cell viability.
  • Antibody-conjugated SLN demonstrated a synergistic effect on cell viability at low concentrations.
  • Higher cellular internalization of antibody-containing SLN was observed in both cell lines, particularly in HER2-positive BT-474 cells.

Conclusions:

  • Antibody-conjugated SLN effectively target and are internalized by breast cancer cells, especially those overexpressing HER2.
  • This targeted delivery system shows potential for enhanced breast cancer therapy.
  • The optimization of nanoparticle conjugation is crucial for effective targeted drug delivery.
Keywords:
Anti-HER2/neuBT-474 cellsCAB51MCF-7 cellsSLN

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