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Genomics of Klebsiella pneumoniae ST16 producing NDM-1, CTX-M-15, and OXA-232

P Espinal1, E Nucleo2, M Caltagirone2

  • 1Department of Infectious Diseases, Istituto Superiore di Sanità, Rome, Italy; Servei de Microbiologia Hospital de la Santa Creu i Sant Pau, Institut d'Investigaciò Biomèdica Sant Pau (IIB Sant Pau), Barcelona, Spain.

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
|November 26, 2018
PubMed
Abstract

Insights

The Sequence Type 16 (ST16) Klebsiella pneumoniae clone has spread globally, exhibiting variations in carbapenemase genes and plasmids. This adaptable lineage poses a significant public health risk.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Carbapenem-resistant Klebsiella pneumoniae (CRKP) poses a global health threat.
  • Sequence Type 16 (ST16) is an emerging lineage of CRKP with international spread.

Purpose of the Study:

  • To genomically characterize the internationally spreading ST16 CRKP clone.
  • To investigate the genetic basis of carbapenem resistance and plasmid content in ST16 strains.

Main Methods:

  • Whole-genome sequencing of Italian ST16 CRKP isolates.
  • Comparative genomic analysis with international ST16 strains.
  • Phylogenetic analysis, core genome MLST, and plasmid content analysis.

Main Results:

  • ST16 CRKP exhibits variability in carbapenemase genes (blaNDM-1, blaOXA-232).
  • Strains harbor multiple plasmids, including those carrying carbapenemase genes.
  • Genomic and plasmid content differences indicate diverse circulating variants, not a single conserved clone.

Conclusions:

  • The ST16 K. pneumoniae clone is globally disseminated.
  • Its genetic diversity and resistance mechanisms suggest it may become a high-risk clone.
Keywords:
Antimicrobial resistanceCarbapenemaseHealthcare-associated infectionsIncFOutbreak

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