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Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
Copper nanoclusters-enhanced chemiluminescence for folic acid and nitrite detection
1Department of Chemistry, Modern College of Humanities and Sciences of Shanxi Normal University, Linfen 041000, Shanxi, PR China; School of Chemistry and Material Science, Shanxi Normal University, Linfen 041004, Shanxi, PR China.
Abstract:
The reaction between diperiodatoargentate(III) (DPA) and folic acid (FA) produced weak chemiluminescence (CL) in acid medium, which was greatly enhanced in the presence of copper nanoclusters (CuNCs). The CL intensity of CuNCs-DPA-FA system increased with the concentration of FA ranging from 0.1 to 10.0 μM. The proposed CL system was applied for the detection of FA in pharmaceutical formulation and human urine samples. Further, the CL signal of CuNCs-DPA-FA system was inhibited by nitrite, and the inhibited CL intensity was proportional to the nitrite concentration in the range of 1.0-80.0 μM. The method was successfully applied to determine nitrite in water, pickled vegetable and sausage samples. A possible CL mechanism was briefly discussed.
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