A visual and sensitive Hg²⁺ detection strategy based on split DNAzyme amplification and peroxidase-like activity of...

A visual and sensitive Hg²⁺ detection strategy was developed based on split DNAzyme amplification and hemin-graphene oxide composites (H-GNs). Two split DNAzyme sequences can form two entire enzyme-strands DNA (E-DNA) by T-Hg²⁺-T interaction. The E-DNA can bind with the loop of molecular beacon (MB) to form Mg²⁺-dependent DNAzyme structure. The formed DNAzyme can circularly cleave the loop of MB, resulting large amount of DNA fragments. The resultant DNA fragments can prevent H-GNs from aggregation by adsorbing on its surface. Consequently, the supernate with large amount of H-GNs shows dark blue color after chromogenic reaction. This strategy shows a linear range from 50 pM to 1200 pM. The limit detection can be low to 33 pM. This strategy provides a visual and enzyme-free amplification mode for quick and sensitive screen of Hg²⁺.