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Related Experiment Video

Updated: Feb 2, 2026

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
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Poly-Target Selection Identifies Broad-Spectrum RNA Aptamers.

Khalid K Alam1, Jonathan L Chang2, Margaret J Lange2

  • 1Department of Biochemistry, University of Missouri, Columbia, MO, USA; Bond Life Sciences Center, University of Missouri, Columbia, MO, USA.

Molecular Therapy. Nucleic Acids
|November 26, 2018
PubMed
Summary
This summary is machine-generated.

Researchers developed a new aptamer selection method to find broad-spectrum antivirals. This approach identified novel aptamers that inhibit diverse primate lentiviral reverse transcriptases (RTs), offering potential for new antiviral therapies.

Keywords:
HIVRNASELEXaptamerbioinformaticsbroad-spectrum inhibitorscross-reactivitydrug resistancereverse transcriptaseselection

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Area of Science:

  • Molecular Biology
  • Virology
  • Biotechnology

Background:

  • Aptamer selections typically yield specific subpopulations.
  • Identifying aptamers with broad-spectrum activity against related targets remains challenging.

Purpose of the Study:

  • To develop a multiplexed poly-target selection strategy to identify aptamers recognizing divergent primate lentiviral reverse transcriptases (RTs).
  • To explore the potential for broad-spectrum antiviral activity from aptamer subpopulations.

Main Methods:

  • Multiplexed poly-target aptamer selection against a panel of primate lentiviral RTs.
  • High-throughput sequencing and coenrichment/codepletion analysis to identify candidate aptamers.
  • Biochemical characterization and viral replication assays to validate aptamer efficacy.

Main Results:

  • Identified dozens of priority aptamer candidates with broad-spectrum binding and inhibitory activity.
  • Validated novel aptamer motifs and rare variants with varying degrees of RT inhibition.
  • Demonstrated suppression of viral replication using phylogenetically diverse RTs.

Conclusions:

  • The poly-target selection and coenrichment/codepletion approach is a generalizable strategy for identifying cross-reactive aptamers.
  • Broad-spectrum aptamers targeting lentiviral RTs can be identified and validated.
  • This method holds promise for developing novel antiviral therapeutics.